Golf courses will be closed under Level 5 restrictions that come into effect at midnight.
Courses from Buncrana to Bundoran will be forced to close.
Golf courses were exempt during the first lockdown.
However, a meeting between Sport Ireland, the Golfing Union of Ireland (GUI) and Irish Ladies Golf Union (ILGU), it has now been confirmed that the clubs will be forced to close for six weeks.
In a joint statement, the bodies said they are still seeking an exemption on ranges and practice facilities.
“Following intensive engagement over the past 48 hours, we have been informed this afternoon by Sport Ireland that, very regrettably, golf clubs must close under the new Level 5 restrictions,” a statement read.
“Essential course maintenance is permitted to continue under Level 5. We have sought clarity on the issue as to whether ranges/practice facilities can remain open for exempted activities. We will update clubs on this as soon as possible.”